IN VITRO ANTIDIABETIC AND ANTIOXIDANT POTENTIAL OF ETHANOLIC WHOLE PLANT EXTRACT OF BIDENS PILOSA THROUGH CARBOHYDRATE DIGESTIVE ENZYME INHIBITION AND GLUCOSE UPTAKE ENHANCEMENT

Abstract

The ethanolic extract of Bidens pilosa (EEBP) was evaluated for its inhibitory effect on carbohydrate digestive enzymes, including α-glucosidase and α-amylase, alongside antioxidant activity and glucose uptake in vitro. The phytochemical screening revealed the presence of flavonoids, phenols, steroids, and terpenoids. Antioxidant studies showed notable DPPH, superoxide, and nitric oxide radical scavenging activities. EEBP significantly inhibited α-glucosidase and α-amylase, with IC50 values of 248.26 µg/ml and 71.35 µg/ml respectively. Glucose uptake studies in rat hemi-diaphragm confirmed the insulin-like potential of EEBP. These results suggest that Bidens pilosa may be a promising natural therapeutic agent for diabetes management.